EpiGenie Epigenetics Product Database

5-Hydroxymethylcytosine (5' hmC) Detection

5-Hydroxymethylcytosine, or 5-hmC is a modified form of 5-mC. It has been shown that 5-hmC and the broadly studied 5-mC cannot be distinguished by sodium bisulfite conversion.

Bisulfite Conversion

Bisulfite Conversion is the use of sodium bisulfite to convert cytosine residues to uracil, but leaving 5-methylcytosine residues unaffected. This allows analysis of DNA methylation patterns.

Bisulfite DNA Amplification

Bisulfite Conversion allows analysis of DNA methylation patterns. After conversion, amplification of treated DNA is the first step in many methylation analysis protocols.

Bisulfite Sequencing Software

Bisulfite Conversion is the use of sodium bisulfite to convert cytosine residues to uracil, while leaving 5-methylcytosine residues unaffected. Sequencing is then used to analyze methylation status.

CpG Island Microarrays

CpG Island Microarrays use probes to short fragments designed to cover CpG Islands throughout the genome for genome-wide measurement of DNA methylation changes.

DNA Methylase Enzymes

DNA Methylases are enzymes that will methylate DNA at certain residues. These can be used create control samples, and/or to help assess the accuracy of a DNA methylation assay.

DNA Methylation Antibodies

DNA Methylation Antibodies bind to either methylated DNA motifs or target specific components involved in the DNA methylation pathway.

DNA Methylation Control DNA

DNA Methylation Control DNA is a DNA sample with a known methylation pattern. This sample can be used as a control in DNA methylation assays.

DNA Methylation Enrichment Kits

DNA Methylation Enrichment Kitsare are optimized systems that bind to methylated DNA. The most common use antibodies or methyl binding proteins (MBPs).

DNA Methylation Sequencing and Pyrosequencing Reagents

After bisulfite conversion and amplification of samples, sequencing of targeted genomic regions can be used to analyze the methylation patterns within that region.

DNA Methylation Sequencing Services

DNA Methylation Sequencing Services harnesses the power of advanced sequencing techniques to identify the sequences of methylated genomic regions within a given sample.

Epigenetic Research Instruments

Epigenetic Research Instruments are devices that automate or simplify epigenetic techniques and protocols.

Genomic DNA Labeling

Genomic DNA Labeling Kits generate labeled genomic DNA targets for use in a variety of array based experiments.

Global DNA Methylation Detection

Global DNA Methylation Detection provides a method to measure global DNA methylation shifts.

HRM Methylation PCR

High resolution melting analysis(HRM)is a real-time PCR-based technique to differentiate converted from unconverted bisulfite-treated DNA to asses DNA methylation

MALDI-TOF Methylation Analysis

MALDI-TOF takes advantage of Mass Spectrometry and Bisulfite Conversions to determine the specific pattern of DNA methylation of CpG sites.

Methylation Dependent Restriction Enzymes

Methylation Dependent Restriction Enzymes cleave DNA at specific methylated-cytosine residues in CpG motifs which have a methyl group, leaving unmethylated DNA intact.

Methylation Sensitive Restriction Enzymes

Methylation Sensitive Restriction Enzymes cleave DNA at specific methylated-cytosine residues in CpG motifs which have lost their methyl group, leaving only hypermethylated DNA intact.

Methylation Specific PCR-MSP

Methylation Specific PCR (MSP) analysis uses bisulfite-treated DNA but avoids the need to sequence the area of interest.

Methylation Specific qPCR

Methylation Specific qPCR is based on Methylation Specific PCR (MSP), but provides a quantitative analysis using real-time PCR.

Methylight Assays

Real-Time Methylation PCR-Methylight assays are used to analyze DNA methylation. MethyLight Assays enable discrimination between unmethylated and methylated sites in the same sample.

Methyltransferase Enzymes

Methyltransferase enzymes can be used to methylate sample DNA, or be used to study mechanisms of DNA methylation.

MSRE Based DNA Methylation qPCR

MSRE based DNA Methylation qPCR uses restriction enzymes (MSREs)to digest DNA at specific sites according to methylation status, which can then be measured by qPCR.

Promoter Microarrays

Promoter Arrays concentrate on targeting promoter regions in the genome and are used in ChIP-chip, MeDIP-chip and promoter sequence enrichment protocols.

Tiling Microarrays

Tiling arrays use short fragments designed to cover the entire genome or areas of the genome in gene expression analysis, transcriptome mapping, ChIP-chip, MeDIP-chip,DNase Chip studies and array CGH.

Whole Genome Amplification

Whole genome amplification (WGA) is a group of procedures that allow amplification to occur at many locations in an unknown genome.

ChIP Kits and Reagents

Chromatin immunoprecipitation (ChIP) is a tool for identifying proteins, including histone and non-histone proteins, associated with specific regions of the genome by using antibodies.

ChIP PCR Kits

Chromatin Immunoprecipitation (ChIP) PCR Assays are PCR primer sets or arrays designed to measure genomic DNA enrichment among ChIP-purified DNA samples.

ChIP-Chip and ChIP-Seq Services

ChIP-chip & ChIP-seq combine chromatin IP with microarray or sequencing to investigate interactions between proteins and DNA in vivo. These experiments can be run for you as a service.

ChIP-Chip Microarrays

ChIP-chip is a technique that combines chromatin immunoprecipitation with microarray technology. Like regular ChIP, ChIP-on-chip is used to investigate interactions between proteins and DNA in vivo.

Chromatin Antibodies

Antibodies to chromatin related proteins. Often used in chromatin immunoprecipitation (ChIP) experiments.

Epigenetic Research Instruments

Epigenetic Research Instruments are devices that automate or simplify epigenetic techniques and protocols.

Genomic DNA Labeling

Genomic DNA Labeling Kits generate labeled genomic DNA targets for use in a variety of array based experiments.

Histone Acetyltransferase (HAT) Assays

Histone acetyltransferases (HAT) are enzymes that acetylate lysine amino acids on histone proteins and play a crucial role in transcription, differentiation, and proliferation.

Histone Deacetylase (HDAC) Assays

Assays to measure and detect to activity of histone deacytlase enzymes (HDAC)

Histone Deacetylase Antibodies

Histone deacetylase (HDAC) enzymes influence transcription by selectively deacetylating lysine residues. HDAC Antibodies can be used to monitor cellular levels of HDAC enzymes.

Histone Demethylase Antibodies

Antibodies to histone demethylase enzymes

Histone H1 Antibodies

Histone H1 Antibodies bind to Histone H1.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.

Histone H2A Antibodies

Histone H2A Antibodies bind to Histone H2A.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.

Histone H2B Antibodies

Histone H2B Antibodies bind to Histone H2B. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.

Histone H3 Antibodies

Histone H3 Antibodies bind to Histone H3. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.

Histone H4 Antibodies

Histone H4 Antibodies bind to Histone H4. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.

Histone Methyltransferase Antibodies

Antibodies to histone methyltransferase enzymes

Histone Peptide Arrays

Histone Peptide Arrays provide a tool to screen antibodies, enzymes and proteins for cross-reactivity or binding interactions with histones and their modifications.

Histones

Recombinant histones used to study function of histones and their variants.

Promoter Microarrays

Promoter Arrays concentrate on targeting promoter regions in the genome and are used in ChIP-chip, MeDIP-chip and promoter sequence enrichment protocols.

Protein Arginine Methylation

Protein Arginine Methylation is a form of epigenetic regulation that effects gene expression via post-translational modifications and the placement of methyl marks on histones and other proteins.

Tiling Microarrays

Tiling arrays use short fragments designed to cover the entire genome or areas of the genome in gene expression analysis, transcriptome mapping, ChIP-chip, MeDIP-chip,DNase Chip studies and array CGH.

Whole Genome Amplification

Whole genome amplification (WGA) is a group of procedures that allow amplification to occur at many locations in an unknown genome.

Epigenetic Research Instruments

Epigenetic Research Instruments are devices that automate or simplify epigenetic techniques and protocols.

Exosome Isolation Kits

Exosome Isolation Kits enable the collection and processing of exosomes for analysis.

miRNA Analysis Services

miRNA Analysis Services allow you to take advantage of the expertise companies have developed in the miRNA field to profile miRNA expression in your samples.

miRNA and RBP Antibodies

Antibodies to miRNA related and RNA binding proteins. These can be used in detection or pulldown experiments like RNA Binding Protein Immunoprecipitation (RIP)

miRNA Bead-Based Profiling

miRNA bead-based profiling makes use of magnetic bead technology to assess the miRNA signature of a sample.

miRNA Cloning

miRNA Cloning Kits are used for cloning and identifying miRNAs within a cell or tissue.

miRNA Inhibition

miRNA Inhibition techniques are used in functional miRNA studies to assess miRNA regulation after it has been inhibited in a model system.

miRNA Linear Amplification

miRNA linear amplification approaches increase the overall target representation of miRNAs in a given sample.

miRNA Microarray Labeling

miRNA Microarray Labeling kits enable you to add fluorescence to the end of miRNAs, or other small RNAs, in your sample so that you can profile their relative abundance by microarray.

miRNA Microarrays

miRNA Microarrays allow a researcher to profile the expression of virtually every miRNA in a sample simultaneously on one array slide.

miRNA Over-Expression

miRNA Over-Expression techniques are used in functional miRNA studies to assess miRNA regulation after it has been introduced into a model system.

miRNA Purification and Enrichment

miRNA Purification kits make sure you’ll keep the small RNA species in your sample from hitting the waste bin.

miRNA qRT-PCR

miRNA qRT-PCR uses the same techniques as normal qRT-PCR with just a few tweaks to allow the detection, quantification and profiling of miRNAs in your sample in a highly specific way

miRNA Target Identification

miRNA Target Identification tools are designed to help match miRNAs to their targets through functional methods.

RNA Binding Protein Immunoprecipitaion (RIP) Products

RIP can be used to identify specific RNA molecules associated with specific nuclear or cytoplasmic binding proteins.